HLA-DR Rabbit Polyclonal Antibody
cat.: R1510-34
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 29 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human HLA-DR.
Positive control: Raji cell lysate, Daudi cell lysate, D3, human spleen tissue, Jurkat.
Subcellular location: Lysosome membrane, Endosome membrane, Late endosome membrane, trans-Golgi network membrane, Endoplasmic reticulum membrane, Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000-1:5,000
1:50-1:100
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: P01903 Human
Alternative names: DASS-397D15.1 DR alpha chain DR alpha chain precursor DRA_HUMAN DRB1 DRB4 FLJ51114 Histocompatibility antigen HLA DR alpha Histocompatibility antigen HLA-DR alpha HLA class II histocompatibility antigen HLA class II histocompatibility antigen DR alpha chain HLA DR1B HLA DR3B HLA DRA HLA DRA1 HLA DRB1 HLA DRB3 HLA DRB4 HLA DRB5 HLA-DR histocompatibility type HLA-DRA HLADR4B HLADRA1 HLADRB Major histocompatibility complex class II DR alpha Major histocompatibility complex class II DR beta 1 Major histocompatibility complex class II DR beta 3 Major histocompatibility complex class II DR beta 4 Major histocompatibility complex class II DR beta 5 MGC117330 MHC cell surface glycoprotein MHC class II antigen DRA MHC II MLRW OTTHUMP00000029406 OTTHUMP00000029407
Images
R1510-34_1.jpg Fig1: Western blot analysis of HLA-DR on different lysates with Rabbit anti-HLA-DR antibody (R1510-34) at 1/5,000 dilution.

Lane 1: Daudi (Human Burkitt's lymphoma cell) cell lysate
Lane 2: Raji (Human Burkitt's lymphoma cell) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: R1510-34, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 29 kDa
Observed band size: 35 kDa
R1510-34_2.jpg Fig2: ICC staining of HLA-DR in D3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1510-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
R1510-34_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HLA-DR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-34, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1510-34_4.jpg Fig4: Flow cytometric analysis of HLA-DR was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (R1510-34, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.