Peroxiredoxin 2 Rabbit Polyclonal Antibody
cat.: R1510-37
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 22 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PRDX2 aa 149-198 / 198.
Positive control: HeLa cell lysate, PC-12 cell lysate, A549 cell lysate, 293T cell lysate, mouse heart tissue lysate, Hela, SHG-44, SH-SY5Y, human liver tissue, human kidney tissue, mouse brain tissue, mouse prostate tissue, mouse heart tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:1,000-1:5,000
1:50-1:200
1:100-1:500
Uniprot #: SwissProt: P32119 Human | Q61171 Mouse | P35704 Rat
Alternative names: Epididymis secretory sperm binding protein Li 2a HEL S 2a MGC4104 Natural killer cell enhancing factor B Natural killer cell-enhancing factor B Natural Killer Enhancing Factor B NKEF B NKEF-B NKEFB Peroxiredoxin-2 PRDX 2 PRDX2 PRDX2_HUMAN PrP PRX2 PRXII PTX1 TDPX1 Thiol Specific Antioxidant 1 Thiol specific antioxidant protein Thiol-specific antioxidant protein Thioredoxin Dependent Peroxide Reductase 1 Thioredoxin peroxidase 1 Thioredoxin-dependent peroxide reductase 1 Torin TPX1 TSA
Images
R1510-37_1.jpg Fig1: Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody (R1510-37) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: PC-12 cell lysate
Lane 3: A549 cell lysate
Lane 4: 293T cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 15 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
R1510-37_2.png Fig2: Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody (R1510-37) at 1/1,000 dilution.

Lane 1: PC-12 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: Mouse heart tissue lysate (20 µg/Lane)

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-37) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.
R1510-37_3.jpg Fig3: Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody (R1510-37) at 1/5,000 dilution.

Lane 1: HeLa-si NT cell lysate
Lane 2: HeLa-si Peroxiredoxin 2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 3 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-37) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
R1510-37_4.jpg Fig4: ICC staining Peroxiredoxin 2/PRDX2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1510-37_5.jpg Fig5: ICC staining Peroxiredoxin 2/PRDX2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1510-37_6.jpg Fig6: ICC staining Peroxiredoxin 2/PRDX2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1510-37_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.
R1510-37_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.
R1510-37_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.
R1510-37_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.
R1510-37_11.jpg Fig11: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.