PHD2 Rabbit Polyclonal Antibody
cat.: R1510-40
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 46kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PHD2 aa 377-426 / 426. |
| Positive control: | PC-12, human kidney tissue, mouse kidney tissue, human pancreas tissue, mouse brain tissue. |
| Subcellular location: | Cytoplasm, Nucleus |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:50-1:200 1:50-1:200 1:10-1:100 |
| Uniprot #: | SwissProt: Q9GZT9 Human | Q91YE3 Mouse | P59722 Rat |
| Alternative names: | C1ORF12 Chromosome 1 Open Reading Frame 12 DKFZp761F179 ECYT 3 ECYT3 EGL 9 homolog of C. elegans 1 EGL nine (C.elegans) homolog 1 Egl nine homolog 1 (C. elegans) Egl nine homolog 1 Egl nine like protein 1 EGLN 1 Egln1 EGLN1_HUMAN HIF PH2 HIF Prolyl Hydroxylase 2 HIF-PH2 HIF-prolyl hydroxylase 2 HIFP4H 2 HIFPH2 HPH 2 HPH-2 HPH2 Hypoxia inducible factor prolyl hydroxylase 2 Hypoxia-inducible factor prolyl hydroxylase 2 ORF13 P4H2 PHD 2 PhD2 PNAS 118 PNAS 137 Prolyl Hydroxylase Domain Containing Protein 2 Prolyl hydroxylase domain-containing protein 2 Rat Homolog of SM20 SM 20 SM-20 SM20 Zinc finger MYND domain containing protein 6 ZMYND6 |
Images
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Fig1:
Western blot analysis of PHD2 on different lysates with Rabbit anti-PHD2 antibody (R1510-40) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PHD2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 46 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-40) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis on mouse brain lysates using anti-PHD2 rabbit polyclonal antibody. |
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Fig3: Immunocytochemical staining of PC-12 cells using anti-PHD2 rabbit polyclonal antibody. |
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Fig4: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-PHD2 rabbit polyclonal antibody. |
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Fig5: Immunohistochemical analysis of paraffin- embedded human pancreas tissue using anti-PHD2 rabbit polyclonal antibody. |
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Fig6: Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-PHD2 rabbit polyclonal antibody. |
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Fig7: Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-PHD2 rabbit polyclonal antibody. |
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Fig8: Flow cytometric analysis of SH-SY-5Y cells with PHD2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.