Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IHC-P, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 12kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Cytochrome C aa 1-50 / 105. |
Positive control: | Hela, HepG2, 293, MCF-7, human spleen tissue, human kidney tissue, human liver tissue, mouse liver tissue, mouse spleen tissue, mouse heart tissue, mouse kidney tissue. |
Subcellular location: | Mitochondrion intermembrane space |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: P99999 Human | P62897 Mouse | P62898 Rat |
Alternative names: | CYC CYC_HUMAN CYCS Cytochrome c Cytochrome c somatic HCS THC4 |
Fig1: Western blot analysis on mouse brain(1) mouse heart(2) and mouse kidney(3) cell lysates using anti-Cytochrome C rabbit polyclonal antibody. | |
Fig2: Immunocytochemical staining of Hela cells using anti-Cytochrome C rabbit polyclonal antibody. | |
Fig3: Immunocytochemical staining of HepG2 cells using anti-Cytochrome C rabbit polyclonal antibody. | |
Fig4: Immunocytochemical staining of MCF-7 cells using anti-Cytochrome C rabbit polyclonal antibody. |
Fig5: Immunocytochemical staining of 293 cells using anti-Cytochrome C rabbit polyclonal antibody. | |
Fig6:
Immunohistochemical analysis of paraffin- embedded human spleen tissue using anti-Cytochrome C rabbit polyclonal antibody. |
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Fig7:
Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-Cytochrome C rabbit polyclonal antibody. |
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Fig8:
Immunohistochemical analysis of paraffin- embedded mouse liver tissue using anti-Cytochrome C rabbit polyclonal antibody. |
Fig9:
Immunohistochemical analysis of paraffin- embedded mouse spleen tissue using anti-Cytochrome C rabbit polyclonal antibody. |
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Fig10:
Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-Cytochrome C rabbit polyclonal antibody. |
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Fig11:
Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-Cytochrome C rabbit polyclonal antibody. |
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Fig12:
Immunohistochemical analysis of paraffin- embedded mouse heart tissue using anti-Cytochrome C rabbit polyclonal antibody. |
Fig13: Flow cytometric analysis of Hela cells with Cytochrome C antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |