Cytochrome C Rabbit Polyclonal Antibody
cat.: R1510-41
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 12kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cytochrome C aa 1-50 / 105. |
| Positive control: | HeLa cell lysate, L-929 cell lysate, C6 cell lysate, human liver tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, human spleen tissue, human kidney tissue, human liver tissue, mouse liver tissue, mouse spleen tissue, mouse heart tissue, mouse kidney tissue. |
| Subcellular location: | Mitochondrion intermembrane space |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: P99999 Human | P62897 Mouse | P62898 Rat |
| Alternative names: | CYC CYC_HUMAN CYCS Cytochrome c Cytochrome c somatic HCS THC4 |
Images
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Fig1:
Western blot analysis of Cytochrome C on different lysates with Rabbit anti-Cytochrome C antibody (R1510-41) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: L-929 cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: Human liver tissue lysate (40 µg/Lane) Lane 5: Mouse liver tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-41) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin- embedded human spleen tissue using anti-Cytochrome C rabbit polyclonal antibody. |
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Fig3:
Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-Cytochrome C rabbit polyclonal antibody. |
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Fig4:
Immunohistochemical analysis of paraffin- embedded mouse liver tissue using anti-Cytochrome C rabbit polyclonal antibody. |
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Fig5:
Immunohistochemical analysis of paraffin- embedded mouse spleen tissue using anti-Cytochrome C rabbit polyclonal antibody. |
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Fig6:
Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-Cytochrome C rabbit polyclonal antibody. |
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Fig7:
Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-Cytochrome C rabbit polyclonal antibody. |
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Fig8:
Immunohistochemical analysis of paraffin- embedded mouse heart tissue using anti-Cytochrome C rabbit polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.