ABCA1 Rabbit Polyclonal Antibody
cat.: R1510-42
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 0.5ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 254 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ABCA1 aa 1,101-1,312. |
| Positive control: | HepG2 cell lysate, U-87 MG cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate. |
| Subcellular location: | Cell membrane, Endosome. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: O95477 Human | P41233 Mouse | Q80ZB2 Rat |
| Alternative names: | ABC 1 ABC Transporter 1 ABC-1 ABC1 ABCA 1 ABCA1 ABCA1_HUMAN ATP binding Cassette 1 ATP binding cassette sub family A ABC1 member 1 ATP binding cassette sub family A member 1 ATP binding Cassette Transporter 1 ATP-binding cassette 1 ATP-binding cassette sub-family A member 1 ATP-binding cassette transporter 1 CERP Cholesterol efflux regulatory protein FLJ14958 HDLDT1 Membrane bound MGC164864 MGC165011 TD TGD |
Images
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Fig1:
Western blot analysis of ABCA1 on different lysates with Rabbit anti-ABCA1 antibody (R1510-42) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: U-87 MG cell lysate (20 µg/Lane) Lane 3: SH-SY5Y cell lysate (negative) (20 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 254 kDa Observed band size: 254 kDa Exposure time: 15 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-42) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.