ABCA1 Rabbit Polyclonal Antibody
cat.: R1510-42
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 254 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ABCA1 aa 21-70 / 2,261. |
| Positive control: | Rat brain tissue lysate, Jurkat cell lysate, mouse brain tissue lysate, PC-3M, human liver tissue, human pancreas tissue, mouse prostate tissue, mouse pancreas tissue, Jurkat. |
| Subcellular location: | Membrane |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O95477 Human | P41233 Mouse | Q80ZB2 Rat |
| Alternative names: | ABC 1 ABC Transporter 1 ABC-1 ABC1 ABCA 1 ABCA1 ABCA1_HUMAN ATP binding Cassette 1 ATP binding cassette sub family A ABC1 member 1 ATP binding cassette sub family A member 1 ATP binding Cassette Transporter 1 ATP-binding cassette 1 ATP-binding cassette sub-family A member 1 ATP-binding cassette transporter 1 CERP Cholesterol efflux regulatory protein FLJ14958 HDLDT1 Membrane bound MGC164864 MGC165011 TD TGD |
Images
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Fig1:
Western blot analysis of ABCA1 on different lysates with Rabbit anti-ABCA1 antibody (R1510-42) at 1/500 dilution. Lane 1: Rat brain tissue lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Predicted band size: 254 kDa Observed band size: 254 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-42) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunocytochemical staining of PC-3M cells using anti-ABCA1 rabbit polyclonal antibody. |
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Fig3:
Immunocytochemistry analysis of HepG2 cells labeling ABCA1 with Rabbit anti-ABCA1 antibody (R1510-42) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ABCA1 antibody (R1510-42) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. β-tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
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Fig4: Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-ABCA1 rabbit polyclonal antibody |
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Fig5: Immunohistochemical analysis of paraffin- embedded human pancreas tissue using anti-ABCA1 rabbit polyclonal antibody. |
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Fig6: Immunohistochemical analysis of paraffin- embedded mouse prostate tissue using anti-ABCA1 rabbit polyclonal antibody. |
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Fig7: Immunohistochemical analysis of paraffin- embedded mouse pancreas tissue using anti-ABCA1 rabbit polyclonal antibody. |
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Fig8: Flow cytometric analysis of Jurkat cells with ABCA1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.