PDGFR beta Rabbit Polyclonal Antibody
cat.: R1510-44
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 124 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PDGFR beta aa 731-780. |
| Positive control: | U-87 MG (Human glioblastoma cell) cell lysate, C2C12 (Mouse myoblast) cell lysate, C6 (Rat glioma cell) cell lysate, Mouse lung tissue lysate. |
| Subcellular location: | Cell membrane, Cytoplasmic vesicle, Lysosome lumen. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P09619 Human | P05622 Mouse | Q05030 Rat |
| Alternative names: | Beta platelet derived growth factor receptor Beta-type platelet-derived growth factor receptor CD 140B CD140 antigen-like family member B CD140b CD140b antigen IBGC4 IMF1 JTK12 OTTHUMP00000160528 PDGF R beta PDGF-R-beta PDGFR 1 PDGFR PDGFR beta PDGFR1 PDGFRB PGFRB_HUMAN Platelet derived growth factor receptor 1 Platelet derived growth factor receptor beta Platelet derived growth factor receptor beta polypeptide |
Images
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Fig1:
Western blot analysis of PDGFR beta on different lysates with Rabbit anti-PDGFR beta antibody (R1510-44) at 1/5,000 dilution. Lane 1: U-87 MG (Human glioblastoma cell) cell lysate (15 µg/Lane) Lane 2: C2C12 (Mouse myoblast) cell lysate (15 µg/Lane) Lane 3: C6 (Rat glioma cell) cell lysate (15 µg/Lane) Lane 4: Mouse lung tissue lysate (30 µg/Lane) Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1510-44, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 124 kDa Observed band size: 190 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.