HIF-1 alpha Rabbit Polyclonal Antibody
cat.: R1510-5
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 93 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human HIF-1 alpha aa 286-330.
Positive control: Hela, PANC-1, SW480, human tonsil tissue, human colon carcinoma tissue, mouse colon tissue, mouse small intestine tissue, HepG2 cell lysate, A549 cell lysate, Hela cell lysate, PANC-1 cell lysate, SW480 cell lysate, A431 cell lysate, PC-12 cell lysate.
Subcellular location: Cytoplasm, Nucleus, Nucleus speckle
Recommended Dilutions:
  IF-Cell
  IHC-P
  FC
  WB

1:50-1:200
1:50-1:200
1:50-1:100
1:500
Uniprot #: SwissProt: Q16665 Human | Q61221 Mouse | O35800 Rat
Alternative names: ARNT interacting protein ARNT-interacting protein Basic helix loop helix PAS protein MOP1 Basic-helix-loop-helix-PAS protein MOP1 bHLHe78 Class E basic helix-loop-helix protein 78 HIF 1A HIF 1alpha HIF-1-alpha HIF1 A HIF1 Alpha HIF1 HIF1-alpha HIF1A HIF1A_HUMAN Hypoxia inducible factor 1 alpha Hypoxia inducible factor 1 alpha isoform I.3 Hypoxia inducible factor 1 alpha subunit Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor) Hypoxia inducible factor1alpha Hypoxia-inducible factor 1-alpha Member of PAS protein 1 Member of PAS superfamily 1 Member of the PAS Superfamily 1 MOP 1 MOP1 PAS domain-containing protein 8 PASD 8 PASD8
Images
R1510-5_1.jpg Fig1: Immunocytochemical staining of Hela cells using anti-HIF-1 alpha rabbit polyclonal antibody. .
R1510-5_2.jpg Fig2: Immunocytochemical staining of PANC-1 cells using anti-HIF-1 alpha rabbit polyclonal antibody. .
R1510-5_3.jpg Fig3: Immunocytochemical staining of SW480 cells using anti-HIF-1 alpha rabbit polyclonal antibody. .
R1510-5_4.jpg Fig4: Immunohistochemical analysis of paraffin- embedded human tonsil tissue using anti-HIF-1 alpha rabbit polyclonal antibody. .
R1510-5_5.jpg Fig5: Immunohistochemical analysis of paraffin- embedded human colon carcinoma tissue using anti-HIF-1 alpha rabbit polyclonal antibody. .
R1510-5_6.jpg Fig6: Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-HIF-1 alpha rabbit polyclonal antibody. .
R1510-5_7.jpg Fig7: Immunohistochemical analysis of paraffin- embedded mouse small intestine tissue using anti-HIF-1 alpha rabbit polyclonal antibody. .
R1510-5_8.jpg Fig8: Flow cytometric analysis of Hela cells with HIF-1 alpha antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
R1510-5_9.jpg Fig9: Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (R1510-5) at 1/500 dilution.

Lane 1: HepG2 cell lysate
Lane 2: A549 cell lysate
Lane 3: Hela cell lysate
Lane 4: PANC-1 cell lysate
Lane 5: SW480 cell lysate
Lane 6: A431 cell lysate
Lane 7: PC-12 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 93 kDa
Observed band size: 120 kDa

Exposure time: 1 minute;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-5) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.