Calponin-1 Rabbit Polyclonal Antibody
cat.: R1510-7
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 33 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Calponin-1 aa 248-297 / 297. |
| Positive control: | NIH/3T3, mouse smooth muscle tissue, human fetal skeletal muscle tissue, Hela. |
| Subcellular location: | Cytoskeleton, focal adhesion. |
| Recommended Dilutions:
IF-Cell IHC-P FC |
1:50-1:100 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P51911 Human | Q08091 Mouse |
| Alternative names: | Basic calponin Calponin 1 Calponin 1 basic smooth muscle Calponin H1 Calponin H1 smooth muscle Calponin-1 Calponin1 Calponins basic CNN 1 Cnn1 CNN1_HUMAN Epididymis secretory protein Li 14 HEL S 14 Sm Calp SMCC smooth muscle |
Images
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Fig1: ICC staining of Calponin-1 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1510-7, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Calponin-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Calponin-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of Calponin-1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (R1510-7, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.