Beclin 1 Rabbit Polyclonal Antibody
cat.: R1510-8
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human BECN1. |
| Positive control: | Hela, N2A, MCF-7, SHG-44, human liver cancer tissue, human breast tissue, human lung tissue, human kidney tissue, mouse brain tissue, mouse heart tissue, HepG2. |
| Subcellular location: | Cytoplasm, Endosome, Mitochondrion membrane Mitochondrion membrane, Endoplasmic reticulum membrane |
| Recommended Dilutions:
IF-Cell IHC-P FC |
1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q14457 Human | O88597 Mouse |
| Alternative names: | APG6 ATG 6 ATG6 ATG6 autophagy related 6 homolog Bcl-2-interacting protein beclin Beclin 1 (coiled coil moesin like BCL2 interacting protein) Beclin 1 autophagy related Beclin-1 Beclin1 BECN 1 Becn1 BECN1_HUMAN Coiled coil myosin like BCL2 interacting protein Coiled-coil myosin-like BCL2-interacting protein GT197 Protein GT197 VPS 30 VPS30 |
Images
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Fig1: Immunocytochemical staining of N2A cells using anti-BECN1 rabbit polyclonal antibody. |
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Fig2: Immunocytochemical staining of Hela cells using anti-BECN1 rabbit polyclonal antibody. |
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Fig3: Immunocytochemical staining of MCF-7 cells using anti-BECN1 rabbit polyclonal antibody. |
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Fig4: Immunocytochemical staining of SHG-44 cells using anti-BECN1 rabbit polyclonal antibody. |
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Fig5: Immunohistochemical analysis of paraffin- embedded human lung tissue using anti-BECN1 rabbit polyclonal antibody. |
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Fig6: Immunohistochemical analysis of paraffin- embedded human liver cancer tissue using anti-BECN1 rabbit polyclonal antibody. |
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Fig7: Immunohistochemical analysis of paraffin- embedded human breast tissue using anti-BECN1 rabbit polyclonal antibody. |
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Fig8: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-BECN1 rabbit polyclonal antibody. |
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Fig9: Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-BECN1 rabbit polyclonal antibody. |
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Fig10: Immunohistochemical analysis of paraffin- embedded mouse heart tissue using anti-BECN1 rabbit polyclonal antibody. |
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Fig11: Flow cytometric analysis of HepG2 cells with BECN1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.