CCL3 Rabbit Polyclonal Antibody
cat.: R1510-9
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 10 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse CCL3 aa 43-92 / 92. |
| Positive control: | HeLa cell lysate, PANC-1 cell lysate, rat brain tissue lysate, mouse brain tissue lysate, mouse lung tissue lysate, Hela, PANC-1, A549, mouse pancreas tissue, mouse lung tissue, mouse spleen tissue, A549 cell lysate. |
| Subcellular location: | Secreted |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P10147 Human | P10855 Mouse | P50229 Rat |
| Alternative names: | C C motif chemokine 3 CCL 3 CCL3 CCL3_HUMAN Chemokine C C motif ligand 3 G0/G1 switch regulatory protein 19 1 G0/G1 switch regulatory protein 19-1 G0S19 1 G0S19 1 protein Heparin binding chemotaxis protein L2G25B LD78 alpha LD78-alpha(4-69) LD78alpha Macrophage inflammatory protein 1 alpha Macrophage inflammatory protein 1-alpha MIP 1 alpha MIP 1A MIP-1-alpha MIP-1-alpha(4-69) MIP1A PAT 464.1 SCYA 3 SCYA3 SIS alpha SIS beta SIS-beta Small inducible cytokine A3 small inducible cytokine A3 (homologous to mouse Mip-1a) Small-inducible cytokine A3 Tonsillar lymphocyte LD78 alpha protein |
Images
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Fig1:
Western blot analysis of CCL3 on different lysates with Rabbit anti-CCL3 antibody (R1510-9) at 1/1,000 dilution. Lane 1: A549 cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: PANC-1 cell lysate (15 µg/Lane) Lane 4: Rat brain tissue lysate (30 µg/Lane) Lane 5: Mouse brain tissue lysate (30 µg/Lane) Lane 6: Mouse lung tissue lysate (30 µg/Lane) Predicted band size: 10 kDa Observed band size: 20 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-9) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunocytochemical staining of Hela cells using anti-CCL3 rabbit polyclonal antibody. |
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Fig3: Immunocytochemical staining of PANC-1 cells using anti-CCL3 rabbit polyclonal antibody. |
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Fig4: Immunocytochemical staining of A549 cells using anti-CCL3 rabbit polyclonal antibody. |
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Fig5: Immunohistochemical analysis of paraffin- embedded mouse lung tissue using anti-CCL3 rabbit polyclonal antibody. |
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Fig6: Immunohistochemical analysis of paraffin- embedded mouse spleen tissue using anti-CCL3 rabbit polyclonal antibody. |
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Fig7: Immunohistochemical analysis of paraffin- embedded mouse pancreas tissue using anti-CCL3 rabbit polyclonal antibody. |
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Fig8: Flow cytometric analysis of Hela cells with CCL3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.