SIRT6 Rabbit Polyclonal Antibody
cat.: R1511-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SIRT6 aa 1-355. |
| Positive control: | HeLa cell lysate, HCT 116 cell lysate, HepG2 cell lysate, PC-12 cell lysate, HCT 116. |
| Subcellular location: | Nucleus, Chromosome, telomere, Endoplasmic reticulum. |
| Recommended Dilutions:
WB IF-Cell FC |
1:4,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q8N6T7 Human Entrez Gene: 299638 Rat |
| Alternative names: | 2810449N18Rik AI043036 Mono ADP ribosyltransferase sirtuin 6 NAD-dependent protein deacetylase sirtuin-6 Regulatory protein SIR2 homolog 6 Regulatory protein SIR2 homolog SIR2 like 6 SIR2 like protein 6 Sir2 related protein type 6 SIR2-like protein 6 SIR2L6 SIR6_HUMAN SIRT 6 SIRT6 Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) Sirtuin 6 Sirtuin type 6 Sirtuin6 |
Images
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Fig1:
Western blot analysis of SIRT6 on different lysates with Rabbit anti-SIRT6 antibody (R1511-1) at 1/4,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: HepG2 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 45 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-1) at 1/4,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HCT 116 cells labeling SIRT6 with Rabbit anti-SIRT6 antibody (R1511-1) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIRT6 antibody (R1511-1) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HCT 116 cells labeling SIRT6. Cells were fixed and permeabilized. Then stained with the primary antibody (R1511-1, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.