SIRT6 Rabbit Polyclonal Antibody
cat.: R1511-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 39kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human SIRT6. |
| Positive control: | Mouse brain tissue, mouse thymus tissue |
| Subcellular location: | Nucleus, cytoplasm |
| Recommended Dilutions:
IHC-P FC |
1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q8N6T7 Human |
| Alternative names: | 2810449N18Rik AI043036 Mono ADP ribosyltransferase sirtuin 6 NAD-dependent protein deacetylase sirtuin-6 Regulatory protein SIR2 homolog 6 Regulatory protein SIR2 homolog SIR2 like 6 SIR2 like protein 6 Sir2 related protein type 6 SIR2-like protein 6 SIR2L6 SIR6_HUMAN SIRT 6 SIRT6 Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) Sirtuin 6 Sirtuin type 6 Sirtuin6 |
Images
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Fig1: Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-SIRT6 rabbit polyclonal antibody. |
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Fig2: Flow cytometric analysis of Hela cells with SIRT6 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-SIRT6 antibody (R1511-1) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1511-1) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-SIRT6 antibody (R1511-1) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1511-1) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.