Metadherin(LYRIC) Rabbit Polyclonal Antibody
cat.: R1511-10
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 64 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Metadherin aa 18-253. |
| Positive control: | Human skeletal muscle tissue lysate, MCF-7, Hela, N2A, SH-SY5Y, human breast tissue, mouse brain tissue. |
| Subcellular location: | Endoplasmic reticulum membrane. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:100-1:500 1:50-1:200 |
| Uniprot #: | SwissProt: Q86UE4 Human |
| Alternative names: | 3D3 3D3/LYRIC AEG 1 AEG-1 AEG1 Astrocyte elevated gene 1 Astrocyte elevated gene-1 protein LYRIC LYRIC/3D3 LYRIC_HUMAN Lysine rich CEACAM1 associated protein Lysine rich CEACAM1 co isolated protein Lysine-rich CEACAM1 co-isolated protein Metadherin Metastasis adhesion protein MTDH Protein LYRIC |
Images
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Fig1: Western blot analysis of Metadherin(LYRIC) on human skeletal muscle tissue (1) and MCF-7 cell (2) lysate using anti-Metadherin(LYRIC) antibody at 1/1,000 dilution. |
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Fig2: ICC staining Metadherin(LYRIC) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Metadherin(LYRIC) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Metadherin(LYRIC) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Metadherin(LYRIC) antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Metadherin(LYRIC) antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of SH-SY5Y cells with Metadherin(LYRIC) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.