HSP47 Rabbit Polyclonal Antibody
cat.: R1511-11
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 46 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HSP47 aa 72-318 / 418. |
| Positive control: | Rat placenta tissue lysate, Siha cell lysate, NIH/3T3 cell lysate, human placenta tissue. |
| Subcellular location: | Endoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: P50454 Human | P19324 Mouse | P29457 Rat |
| Alternative names: | 47 kDa heat shock protein 47 kDa heat shock protein precursor Arsenic transactivated protein 3 Arsenic-transactivated protein 3 AsTP 3 AsTP3 CBP 1 CBP 2 CBP1 CBP2 Cell proliferation-inducing gene 14 protein Collagen binding protein 1 Collagen binding protein 2 Collagen binding protein Collagen-binding protein 2 Collagen-binding protein Colligen Colligin 1 Colligin 2 Colligin colligin-1 colligin-2 gp46 Heat shock protein 47 Heat-shock protein 47 HGNC 1547 Hsp 47 J6 OI10 PIG 14 PIG14 PPROM Proliferation inducing gene 14 Proliferation inducing gene 14 protein RA A47 RA-A47 Rheumatoid arthritis antigen A 47 rheumatoid arthritis antigen A-47 Rheumatoid arthritis related antigen RA A47 Rheumatoid arthritis-related antigen RA-A47 serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) serine (or cysteine) proteinase inhibitor, clade H (heat shock protein...... |
Images
|
Fig1:
"Western blot analysis of HSP47 on different lysates with Rabbit anti-HSP47 antibody (R1511-11) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Lane 3: C2C12 cell lysate (10 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 46.4 kDa Observed band size: 47 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-11) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature." |
|
Fig2:
Western blot analysis of HSP47 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1511-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat placenta tissue lysate Lane 2: Siha cell lysate Lane 3: NIH/3T3 cell lysate |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HSP47 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1511-11, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.