Thioredoxin Rabbit Polyclonal Antibody
cat.: R1511-16
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 12 kDa, additional band 40 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Thioredoxin aa 66-105 / 105. |
| Positive control: | Hela, HepG2, A431, PC-3M, MCF-7, human liver tissue, human kidney tissue, mouse kidney tissue. |
| Subcellular location: | Nucleus. Cytoplasm. Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50-1:200 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P10599 Human | P10639 Mouse |
| Alternative names: | ADF ATL-derived factor SASP Surface associated sulphydryl protein TRN TXN 1 TXN |
Images
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Fig1:
Western blot analysis of Thioredoxin on different cell lysates using anti-Thioredoxin antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: A431 Lane 4: PC-3M |
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Fig2: ICC staining Thioredoxin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Thioredoxin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Thioredoxin antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Thioredoxin antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Thioredoxin antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Hela cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.