R1511-17

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IF-Cell, IHC-P
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

Predicted band size: 83 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within human Ku80 aa 51-104.

Positive control:

A549, Hela, MCF-7, human liver tissue lysate, human colon cancer tissue, human tonsil tissue, human breast cancer tissue, human kidney tissue.

Subcellular location:

Nucleus. Chromosome.

Recommended Dilutions:
  WB
  IHC-P
  IF-Cell

1:500-1:2.000
1:500-1:5,000
1:500-1:1,000

Uniprot #:

SwissProt: P13010 Human

Alternative names:

86 kDa subunit of Ku antigen ATP dependent DNA helicase 2 subunit 2 ATP dependent DNA helicase II 80 kDa subunit ATP dependent DNA helicase II 86 Kd subunit ATP dependent DNA helicase II ATP-dependent DNA helicase 2 subunit 2 ATP-dependent DNA helicase II 80 kDa subunit CTC box binding factor 85 kDa CTC box-binding factor 85 kDa subunit CTC85 CTCBF DNA repair protein XRCC5 KARP 1 KARP1 Ku 80 Ku autoantigen 80kDa Ku80 Ku86 Ku86 autoantigen related protein 1 KUB 2 KUB2 Lupus Ku autoantigen protein p86 NFIV Nuclear factor IV Thyroid lupus autoantigen Thyroid-lupus autoantigen TLAA X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) X-ray repair cross-complementing protein 5 Xray repair complementing defective repair in Chinese hamster cells 5 XRCC 5 XRCC5 XRCC5_HUMAN

	Fig1: Western blot analysis of Ku80 on different cell lysate using anti-Ku80 antibody at 1/500 dilution. Positive control: Lane 1: A549 Lane 2: Hela Lane 3: MCF-7 Lane 4: Human liver
	Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Ku80 antibody (R1511-17) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (R1511-17) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ku80 antibody. Counter stained with hematoxylin.
	Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Ku80 antibody. Counter stained with hematoxylin.