R1511-18

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IF-Cell, IHC-P
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

Predicted band size: 134 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within human EGFR aa 1107-1180.

Positive control:

Hela, HUVEC, A431, human tonsil tissue, human lung cancer tissue, human liver tissue, human kidney tissue, 2 liver tissue, 2 kidney tissue.

Subcellular location:

Secreted and Cell membrane. Endoplasmic reticulum membrane.

Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:500-1:1000
1:50-1:200
1:50-1:200

Uniprot #:

SwissProt: P00533 Human

Alternative names:

Avian erythroblastic leukemia viral (v erb b) oncogene homolog Cell growth inhibiting protein 40 Cell proliferation inducing protein 61 EGF R EGFR EGFR_HUMAN Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) Epidermal growth factor receptor erb-b2 receptor tyrosine kinase 1 ERBB ERBB1 Errp HER1 mENA NISBD2 Oncogen ERBB PIG61 Proto-oncogene c-ErbB-1 Receptor tyrosine protein kinase ErbB 1 Receptor tyrosine-protein kinase ErbB-1 SA7 Species antigen 7 Urogastrone v-erb-b Avian erythroblastic leukemia viral oncogen homolog wa2 Wa5

	Fig1: Western blot analysis of EGFR on different cell lysate using anti-EGFR antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HUVEC Lane 3: A431
	Fig2: ICC staining EGFR in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-EGFR antibody. Counter stained with hematoxylin.
	Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-EGFR antibody. Counter stained with hematoxylin.

	Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-EGFR antibody. Counter stained with hematoxylin.
	Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EGFR antibody. Counter stained with hematoxylin.
	Fig7: Immunohistochemical analysis of paraffin-embedded 2 kidney tissue using anti-EGFR antibody. Counter stained with hematoxylin.