CLOCK Rabbit Polyclonal Antibody
cat.: R1511-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 95 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse CLOCK 829-855/855. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, mouse kidney tissue lysate, PC-3M, SW480, Hela, 293, human kidney, mouse testis, mouse skeletal muscle tissue, human colon cancer tissue, Hela. |
| Subcellular location: | Cytoplasm, Nucleus |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O15516 Human | O08785 Mouse | Q9WVS9 Rat |
| Alternative names: | bHLHe8 Circadian locomoter output cycles kaput protein Circadian locomoter output cycles protein kaput Circadian Locomotor Output Cycles Kaput Circadium Locomotor Output Cycles Kaput Class E basic helix-loop-helix protein 8 CLOCK Clock circadian regulator Clock homolog Clock protein CLOCK_HUMAN hCLOCK KIAA0334 |
Images
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Fig1:
Western blot analysis of CLOCK on different lysates with Rabbit anti-CLOCK antibody (R1511-2) at 1/2,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: MCF7 cell lysate (15 µg/Lane) Lane 3: HEK-293 cell lysate (15 µg/Lane) Lane 4: NIH/3T3 cell lysate (15 µg/Lane) Lane 5: PC-12 cell lysate (15 µg/Lane) Lane 6: Mouse brain tissue lysate (30 µg/Lane) Lane 7: Mouse kidney tissue lysate (30 µg/Lane) Predicted band size: 95 kDa Observed band size: 95 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-2) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunocytochemical staining of PC-3M cells using anti-CLOCK rabbit polyclonal antibody. |
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Fig3: Immunocytochemical staining of SW480 cells using anti-CLOCK rabbit polyclonal antibody. |
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Fig4: Immunocytochemical staining of Hela cells using anti-CLOCK rabbit polyclonal antibody. |
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Fig5: Immunocytochemical staining of 293 cells using anti-CLOCK rabbit polyclonal antibody. |
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Fig6: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-CLOCK rabbit polyclonal antibody. |
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Fig7: Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-CLOCK rabbit polyclonal antibody. |
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Fig8: Immunohistochemical analysis of paraffin- embedded mouse skeletal muscle tissue using anti-CLOCK rabbit polyclonal antibody. |
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Fig9: Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-CLOCK rabbit polyclonal antibody. |
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Fig10: Flow cytometric analysis of Hela cells with CLOCK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.