Ribonuclease 3 Rabbit Polyclonal Antibody
cat.: R1511-20
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein within human Ribonuclease 3. |
| Positive control: | Human spleen tissue, U-937 cell lysates. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P ELISA |
1:1,000 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: P12724 Human |
| Alternative names: | Cytotoxic ribonuclease ECP ECP_HUMAN Eosinophil cationic protein OTTHUMP00000164017 Ribonuclease 3 Ribonuclease, RNase A family, 3 RNase 3 RNASE3 RNS3 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Ribonuclease 3 antibody (R1511-20) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1511-20) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Western blot analysis of Ribonuclease 3 on U-937 cell lysates with Rabbit anti-Ribonuclease 3 antibody (R1511-20) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-20) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.