Ribonuclease 3 Rabbit Polyclonal Antibody
cat.: R1511-22
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Ribonuclease 3 aa 111-160 / 160 |
| Positive control: | U-937 cell lysates. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P ELISA |
1:1,000 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: P12724 Human |
| Alternative names: | Cytotoxic ribonuclease ECP ECP_HUMAN Eosinophil cationic protein OTTHUMP00000164017 Ribonuclease 3 Ribonuclease, RNase A family, 3 RNase 3 RNASE3 RNS3 |
Images
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Fig1:
Western blot analysis of Ribonuclease 3 on U-937 cell lysates with Rabbit anti-Ribonuclease 3 antibody (R1511-22) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-22) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.