Human IgM Rabbit Polyclonal Antibody
cat.: R1511-23
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 70/30 kDa |
| Isotype: | IgG |
| Immunogen: | Human IgM from Human serum (Sigma#I8260). |
| Positive control: | Raji, human plasma, human tonsil tissue, human spleen tissue. |
| Subcellular location: | Cell Membrane and Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P01871 Human |
| Alternative names: | Immunoglobin heavy chain constant region mu edit item name - Immunoglobin heavy chain mu constant region Immunoglobin heavy chain constant region mu AGM1 Constant region of heavy chain of IgM DKFZp686I15196 DKFZp686I15212 FLJ00385 Ig mu chain C region IGHM IgM heavy chain constant region Immunoglobin heavy constant mu Immunoglobulin mu MGC104996 MGC52291 MU VH |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Human IgM antibody. Counter stained with hematoxylin. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Human IgM antibody. Counter stained with hematoxylin. |
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Fig3:
Western blot analysis of Human IgM on different lysates with Rabbit anti-Human IgM antibody (R1511-23) at 1/2,000 dilution. Lane 1:Raji (human Burkitt's lymphoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 180 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1511-23, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 70 kDa Observed band size: 75 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.