SHP-1(PTPN6) Rabbit Polyclonal Antibody
cat.: R1511-24
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal Human SHP-1 aa 550-595 595. |
| Positive control: | Mouse spleen tissue lysate, A549, Hela, Jurkat, rat spinal cord tissue, human tonsil tissue, human spleen tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P29350 Human | P29351 Mouse | P81718 Rat |
| Alternative names: | 70Z-SHP EC 3.1.3.48 HCP HCPH Hematopoietic cell phosphatase Hematopoietic cell protein tyrosine phosphatase Hematopoietic cell protein-tyrosine phosphatase HPTP1C Protein tyrosine phosphatase 1C Protein tyrosine phosphatase non receptor type 6 Protein tyrosine phosphatase SHP1 Protein-tyrosine phosphatase 1C protein-tyrosine phosphatase SHP 1 Protein-tyrosine phosphatase SHP-1 PTN6_HUMAN PTP 1C PTP-1C PTP1C Ptpn6 SH PTP 1 SH PTP1 SH-PTP1 SHP 1 SHP 1L SHP1 SHP1L tyrosine protein phosphatase non receptor type 6 Tyrosine-protein phosphatase non-receptor type 6 |
Images
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Fig1: Western blot analysis of SHP1 on mouse spleen tissue lysate using anti- SHP1 antibody at 1/5,000 dilution. |
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Fig2: ICC staining SHP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining SHP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- SHP1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti- SHP1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti- SHP1 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Jurkat cells with SHP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.