R1511-24

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

Predicted band size: 68 kDa

Isotype:

IgG

Immunogen:

Recombinant protein within C-terminal Human SHP-1 aa 550-595 595.

Positive control:

Mouse spleen tissue lysate, A549, Hela, Jurkat, rat spinal cord tissue, human tonsil tissue, human spleen tissue.

Subcellular location:

Cytoplasm. Nucleus.

Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:1,000-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200

Uniprot #:

SwissProt: P29350 Human | P29351 Mouse | P81718 Rat

Alternative names:

70Z-SHP EC 3.1.3.48 HCP HCPH Hematopoietic cell phosphatase Hematopoietic cell protein tyrosine phosphatase Hematopoietic cell protein-tyrosine phosphatase HPTP1C Protein tyrosine phosphatase 1C Protein tyrosine phosphatase non receptor type 6 Protein tyrosine phosphatase SHP1 Protein-tyrosine phosphatase 1C protein-tyrosine phosphatase SHP 1 Protein-tyrosine phosphatase SHP-1 PTN6_HUMAN PTP 1C PTP-1C PTP1C Ptpn6 SH PTP 1 SH PTP1 SH-PTP1 SHP 1 SHP 1L SHP1 SHP1L tyrosine protein phosphatase non receptor type 6 Tyrosine-protein phosphatase non-receptor type 6

	Fig1: Western blot analysis of SHP1 on mouse spleen tissue lysate using anti- SHP1 antibody at 1/5,000 dilution.
	Fig2: ICC staining SHP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining SHP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- SHP1 antibody. Counter stained with hematoxylin.

	Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti- SHP1 antibody. Counter stained with hematoxylin.
	Fig6: Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti- SHP1 antibody. Counter stained with hematoxylin.
	Fig7: Flow cytometric analysis of Jurkat cells with SHP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).