FPR2 Rabbit Polyclonal Antibody
cat.: R1511-25
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse FPR2 aa 163-250. |
| Positive control: | Mouse lung tissue lysates, Hela, Jurkat, PC-3M, HL-60, mouse lung tissue, human lung tissue, mouse testis tissue, human spleen tissue, mouse spleen tissue |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P25090 Human | O88536 Mouse |
| Alternative names: | ALXR FMLP-R-I FMLP-R-II FMLP-related receptor I FMLPX Formyl peptide receptor 2 Formyl peptide receptor related Formyl peptide receptor-like 1 FPR2 FPR2_HUMAN FPR2A FPRH1 FPRH2 FPRL1 HM63 Lipoxin A4 receptor LXA4 receptor LXA4R N-formyl peptide receptor 2 RFP |
Images
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Fig1:
Western blot analysis on different lysates using anti-FPR2 rabbit polyclonal antibody. Positive control: Lane 1: Jurkat Lane 2: HL-60 |
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Fig2:
Western blot analysis of FPR2 on mouse lung tissue lysates with Rabbit anti-FPR2 antibody (R1511-25) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 55 kDa Exposure time: 10 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-25) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunocytochemical staining of Hela cells using anti-FPR2 rabbit polyclonal antibody. |
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Fig4: Immunocytochemical staining of PC-3M cells using anti-FPR2 rabbit polyclonal antibody. |
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Fig5: Immunohistochemical analysis of paraffin- embedded human lung tissue using anti-FPR2 rabbit polyclonal antibody. |
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Fig6: Immunohistochemical analysis of paraffin- embedded human spleen tissue using anti-FPR2 rabbit polyclonal antibody. |
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Fig7: Immunohistochemical analysis of paraffin- embedded mouse lung tissue using anti-FPR2 rabbit polyclonal antibody. |
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Fig8: Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-FPR2 rabbit polyclonal antibody. |
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Fig9: Immunohistochemical analysis of paraffin- embedded mouse spleen tissue using anti-FPR2 rabbit polyclonal antibody. |
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Fig10: Flow cytometric analysis of Jurkat cells with FPR2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.