Myosin light chain kinase / MLCK Rabbit Polyclonal Antibody
cat.: R1511-26
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell, IHC-P, FC, WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 211 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein with Human MLCK aa 230-470 / 1,914. |
| Positive control: | MCF-7, SW480, mouse colon tissue, mouse smooth muscle tissue, human kidney tissue lysates. |
| Subcellular location: | Cytoplasm, Cell projection, Cleavage furrow |
| Recommended Dilutions:
IF-Cell IHC-P FC WB |
1:50-1:200 1:50-1:200 1:10-1:100 1:1,000 |
| Uniprot #: | SwissProt: Q15746 Human | Q6PDN3 Mouse |
| Alternative names: | caMLCK cardiac MLCK Cardiac MyBP C associated Ca/CaM kinase Cardiac-MyBP-C-associated Ca/CaM kinase MLC kinase MLCK MLCK2 Mylk3 MYLK3_HUMAN Myosin light chain kinase 3 Putative myosin light chain kinase 3 |
Images
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Fig1: Immunocytochemical staining of MCF-7 cells using anti-MYLK rabbit polyclonal antibody. |
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Fig2: Immunocytochemical staining of SW480 cells using anti-MYLK rabbit polyclonal antibody. |
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Fig3: Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-MYLK rabbit polyclonal antibody. |
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Fig4: Immunohistochemical analysis of paraffin- embedded mouse smooth muscle tissue using anti-MYLK rabbit polyclonal antibody. |
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Fig5: Flow cytometric analysis of MCF-7 cells with MYLK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
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Fig6:
Western blot analysis of Myosin light chain kinase / MLCK on human kidney tissue lysates with Rabbit anti-Myosin light chain kinase / MLCK antibody (R1511-26) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 211 kDa Observed band size: 120 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-26) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.