SIRT3 Rabbit Polyclonal Antibody
cat.: R1511-3
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 44 kDa
Isotype: IgG
Immunogen: Recombinant protein within human SIRT3 aa 51-399 / 399.
Positive control: PANC-1 cell lysate, HepG2 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate.
Subcellular location: Mitochondrion matrix
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: Q9NTG7 Human | Q8R104 Mouse
Entrez Gene: 293615 Rat
Alternative names: hSIRT 3 hSIRT3 Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase NAD dependent deacetylase sirtuin 3 mitochondrial NAD-dependent protein deacetylase sirtuin-3, mitochondrial Regulatory protein SIR2 homolog 3 Silent mating type information regulation 2 S.cerevisiae homolog 3 Sir 2 like 3 SIR 2 like protein 3 SIR 3 SIR2 L3 Sir2 like 3 SIR2 like protein 3 SIR2-like protein 3 SIR2L3 SIR3_HUMAN SIRT 3 SIRT3 Sirtuin 3 Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) Sirtuin type 3 Sirtuin3
Images
R1511-3_1.jpg Fig1: Western blot analysis of SIRT3 on different lysates with Rabbit anti-SIRT3 antibody (R1511-3) at 1/2,000 dilution.

Lane 1: PANC-1 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: Mouse liver tissue lysate (40 µg/Lane)
Lane 4: Rat liver tissue lysate (40 µg/Lane)

Predicted band size: 44 kDa
Observed band size: 28 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-3) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.