Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 44 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human SIRT3 aa 51-399 / 399. |
Positive control: | PANC-1 cell lysate, HepG2 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate. |
Subcellular location: | Mitochondrion matrix |
Recommended Dilutions:
WB |
1:2,000 |
Uniprot #: | SwissProt: Q9NTG7 Human | Q8R104 Mouse Entrez Gene: 293615 Rat |
Alternative names: | hSIRT 3 hSIRT3 Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase NAD dependent deacetylase sirtuin 3 mitochondrial NAD-dependent protein deacetylase sirtuin-3, mitochondrial Regulatory protein SIR2 homolog 3 Silent mating type information regulation 2 S.cerevisiae homolog 3 Sir 2 like 3 SIR 2 like protein 3 SIR 3 SIR2 L3 Sir2 like 3 SIR2 like protein 3 SIR2-like protein 3 SIR2L3 SIR3_HUMAN SIRT 3 SIRT3 Sirtuin 3 Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) Sirtuin type 3 Sirtuin3 |
Fig1:
Western blot analysis of SIRT3 on different lysates with Rabbit anti-SIRT3 antibody (R1511-3) at 1/2,000 dilution. Lane 1: PANC-1 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (40 µg/Lane) Lane 4: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 44 kDa Observed band size: 28 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-3) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |