IFNAR1 Rabbit Polyclonal Antibody
cat.: R1511-30
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 64 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IFNAR1 aa 28-227/557. |
| Positive control: | SW620, K562, human liver. |
| Subcellular location: | Late endosome, Lysosome, Cell membrane. |
| Recommended Dilutions:
IF-Cell IHC-P FC |
1:50-1:100 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P17181 Human |
| Alternative names: | Alpha type antiviral protein Antiviral protein, alpha-type Antiviral protein, beta-type AVP Beta type antiviral protein CRF2-1 Cytokine receptor class-II member 1 Cytokine receptor family 2 member 1 IFN alpha REC IFN alpha receptor IFN alpha/beta Receptor alpha IFN beta receptor IFN Interferon-beta receptor IFN-alpha/beta receptor 1 IFN-R-1 IFNAR Ifnar1 IFNBR IFRC INAR1_HUMAN Interferon (alpha beta and omega) receptor 1 interferon alpha and beta receptor subunit 1 Interferon alpha/beta receptor 1 Interferon alpha/beta receptor alpha chain Interferon beta receptor 1 interferon receptor 1 Interferon-alpha receptor Type I interferon receptor 1 |
Images
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Fig1: ICC staining of IFNAR1 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1511-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-IFNAR1 antibody (R1511-30) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1511-30) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of IFNAR1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (R1511-30, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.