| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Bad aa 1-50 / 168. |
| Positive control: | HepG2, SK-BR-3, SW480, human breast carcinoma tissue, MCF-7 |
| Subcellular location: | Mitochondrion outer membrane, Cytoplasm |
| Recommended Dilutions:
IF-Cell IHC-P FC |
1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q92934 Human |
| Alternative names: | AI325008 BAD BAD_HUMAN BBC 2 BBC2 BBC6 Bcl 2 Antagonist of Cell Death Bcl 2 Binding Component 6 BCL X / BCL 2 Binding Protein BCL X Binding Protein Bcl XL/Bcl 2 Associated Death Promoter Bcl-2-binding component 6 Bcl-2-like protein 8 Bcl-XL/Bcl-2-associated death promoter Bcl2 antagonist of cell death BCL2 antagonist of cell death protein BCL2 associated agonist of cell death Bcl2 Associated Death Promoter BCL2 binding component 6 BCL2 binding protein Bcl2 Like 8 Protein Bcl2-L-8 BCL2L8 Proapoptotic BH3 Only Protein |
|
Fig1: Immunocytochemical staining of HepG2 cells using anti-Bad rabbit polyclonal antibody. |
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Fig2: Immunocytochemical staining of SK-BR-3 cells using anti-Bad rabbit polyclonal antibody. |
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Fig3: Immunocytochemical staining of SW480 cells using anti-Bad rabbit polyclonal antibody. |
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Fig4: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-Bad rabbit polyclonal antibody. |
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Fig5: Flow cytometric analysis of MCF-7 cells with Bad antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |