Bad Rabbit Polyclonal Antibody
cat.: R1511-32
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Isotype: IgG
Immunogen: Synthetic peptide within Human Bad aa 1-50 / 168.
Positive control: HepG2, SK-BR-3, SW480, human breast carcinoma tissue, MCF-7
Subcellular location: Mitochondrion outer membrane, Cytoplasm
Recommended Dilutions:
  IF-Cell
  IHC-P
  FC

1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q92934 Human
Alternative names: AI325008 BAD BAD_HUMAN BBC 2 BBC2 BBC6 Bcl 2 Antagonist of Cell Death Bcl 2 Binding Component 6 BCL X / BCL 2 Binding Protein BCL X Binding Protein Bcl XL/Bcl 2 Associated Death Promoter Bcl-2-binding component 6 Bcl-2-like protein 8 Bcl-XL/Bcl-2-associated death promoter Bcl2 antagonist of cell death BCL2 antagonist of cell death protein BCL2 associated agonist of cell death Bcl2 Associated Death Promoter BCL2 binding component 6 BCL2 binding protein Bcl2 Like 8 Protein Bcl2-L-8 BCL2L8 Proapoptotic BH3 Only Protein
Images
R1511-32_1.jpg Fig1: Immunocytochemical staining of HepG2 cells using anti-Bad rabbit polyclonal antibody.
R1511-32_2.jpg Fig2: Immunocytochemical staining of SK-BR-3 cells using anti-Bad rabbit polyclonal antibody.
R1511-32_3.jpg Fig3: Immunocytochemical staining of SW480 cells using anti-Bad rabbit polyclonal antibody.
R1511-32_4.jpg Fig4: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-Bad rabbit polyclonal antibody.
R1511-32_5.jpg Fig5: Flow cytometric analysis of MCF-7 cells with Bad antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.