Hsc70 Rabbit Polyclonal Antibody
cat.: R1511-6
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 71 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human HSP73. |
| Positive control: | HepG2, Hela, NIH-3T3, F9, PC-12, MCF-7, SW480, human breast tissue, human kidney tissue, mouse colon tissue, mouse kidney tissue. |
| Subcellular location: | Nucleus. Cytoplasm. Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P11142 Human | P63017 Mouse | P63018 Rat |
| Alternative names: | 2410008N15Rik Constitutive heat shock protein 70 Epididymis luminal protein 33 Epididymis secretory sperm binding protein Li 72p Heat shock 70 kDa protein 8 Heat shock 70kD protein 10 Heat shock 70kD protein 8 Heat shock 70kDa protein 8 Heat shock cognate 71 kDa protein Heat shock cognate protein 54 Heat shock cognate protein 71 kDa Heat shock protein 8 Heat shock protein A8 Heat shock protein family A (Hsp70) member 8 Heat-shock70-KD protein 10, formerly HEL 33 HEL S 72p HSC54 HSC71 Hsc73 HSP71 HSP73 HSP7C_HUMAN HSPA10 HSPA8 LAP1 Lipopolysaccharide associated protein 1 LPS associated protein 1 LPS associated protein MGC102007 MGC106514 MGC114311 MGC118485 MGC131511 MGC29929 N-myristoyltransferase inhibitor protein 71 NIP71 |
Images
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Fig1: ICC staining HSC70 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining HSC70 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining HSC70 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti- HSC70 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- HSC70 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti- HSC70 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- HSC70 antibody. Counter stained with hematoxylin. |
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Fig8:
Western blot analysis of Hsc70 on different lysates with Rabbit anti-Hsc70 antibody (R1511-6) at 1/2,000 dilution. Lane 1: HepG2 (Human hepatocellular carcinoma cell) cell lysate Lane 2: Hela (Human cervical adenocarcinoma cell) cell lysate Lane 3: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 6 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1511-6, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 71 kDa Observed band size: 71 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.