CYP2E1 Rabbit Polyclonal Antibody
cat.: R1511-7
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CYP2E1 aa 406-449. |
| Positive control: | MCF-7, NIH-3T3, mouse kidney tissue lysate, C2C12, HepG2, mouse liver tissue. |
| Subcellular location: | Endoplasmic reticulum membrane. Microsome membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P05181 Human | Q05421 Mouse |
| Alternative names: | 4-nitrophenol 2-hydroxylase CP2E1_HUMAN CPE1 CYP2E Cyp2e1 CYPIIE1 Cytochrome P450 2E1 Cytochrome P450 isozyme 3A Cytochrome P450 LM3A Cytochrome P450, family 2, subfamily e, polypeptide 1 cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 Cytochrome P450, subfamily IIE Cytochrome P450-ALC Cytochrome P450-J Cytochrome P450RLM6 EC 1.14.13.- EC 1.14.13.n7 Ethanol-inducible P450 Flavoprotein-linked monooxygenase MGC133529 Microsomal monooxygenase OTTHUMP00000020813 OTTHUMP00000046571 P450 ALC P450-J P450C2E P450J P450RLM6 Xenobiotic monooxygenase |
Images
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Fig1: ICC staining Cytochrome P450 2E1 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining Cytochrome P450 2E1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytochrome P450 2E1 antibody. Counter stained with hematoxylin. |
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Fig4:
Western blot analysis of CYP2E1 on different lysates with Rabbit anti-CYP2E1 antibody (R1511-7) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 62 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1511-7, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 57 kDa Observed band size: 50 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.