R1511-7

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IF-Cell, IHC-P
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

57 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within human Cytochrome P450 2E1 aa 406-449.

Positive control:

MCF-7, NIH-3T3, mouse kidney tissue lysate, C2C12, HepG2, mouse liver tissue.

Subcellular location:

Endoplasmic reticulum membrane. Microsome membrane.

Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:1000
1:50-1:200
1:50-1:200

Uniprot #:

SwissProt: P05181 Human | Q05421 Mouse

Alternative names:

4-nitrophenol 2-hydroxylase CP2E1_HUMAN CPE1 CYP2E Cyp2e1 CYPIIE1 Cytochrome P450 2E1 Cytochrome P450 isozyme 3A Cytochrome P450 LM3A Cytochrome P450, family 2, subfamily e, polypeptide 1 cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 Cytochrome P450, subfamily IIE Cytochrome P450-ALC Cytochrome P450-J Cytochrome P450RLM6 EC 1.14.13.- EC 1.14.13.n7 Ethanol-inducible P450 Flavoprotein-linked monooxygenase MGC133529 Microsomal monooxygenase OTTHUMP00000020813 OTTHUMP00000046571 P450 ALC P450-J P450C2E P450J P450RLM6 Xenobiotic monooxygenase

	Fig1: Western blot analysis of Cytochrome P450 2E1 on different lysates using anti-Cytochrome P450 2E1 antibody at 1/1000 dilution. Positive control: Lane 1: MCF-7 Lane 2: NIH-3T3 Lane 3: Mouse kidney tissue Lane 4: siHa
	Fig2: ICC staining Cytochrome P450 2E1 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining Cytochrome P450 2E1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytochrome P450 2E1 antibody. Counter stained with hematoxylin.