ESD Rabbit Polyclonal Antibody
cat.: R1511-9
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 31 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human ESD aa 1-282 / 282.
Positive control: K562 cell lysate, mouse intestine tissue lysate, Hela, LOVO, SW480, human prostate tissue, human pancreas tissue, mouse colon tissue.
Subcellular location: Cytoplasm, Cytoplasmic vesicle.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:500-1:2000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P10768 Human | Q9R0P3 Mouse
Alternative names: EC 3.1.2.12 Es-10 Es10 ESD ESTD_HUMAN Esterase 10 Esterase D Esterase D formylglutathione hydrolase FGH FGH, included FLJ11763 Methylumbelliferyl acetate deacetylase MGC139609 OTTHUMP00000018374 OTTHUMP00000040929 S formylglutathione hydrolase S-formylglutathione hydrolase S-formylglutathione hydrolase, included Sid 478 sid478
Images
R1511-9_1.jpg Fig1: Western blot analysis of ESD on K562 cell (1) and mouse intestine tissue (2) lysate using anti-ESD antibody at 1/1,000 dilution.
R1511-9_2.jpg Fig2: ICC staining ESD in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1511-9_3.jpg Fig3: ICC staining ESD in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1511-9_4.jpg Fig4: ICC staining ESD in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1511-9_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-ESD antibody. Counter stained with hematoxylin.
R1511-9_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ESD antibody. Counter stained with hematoxylin.
R1511-9_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-e=ESD antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.