Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IF-Cell, IHC-P |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 31 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human ESD aa 1-282 / 282. |
Positive control: | K562 cell lysate, mouse intestine tissue lysate, Hela, LOVO, SW480, human prostate tissue, human pancreas tissue, mouse colon tissue. |
Subcellular location: | Cytoplasm, Cytoplasmic vesicle. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2000 1:50-1:200 1:50-1:200 |
Uniprot #: | SwissProt: P10768 Human | Q9R0P3 Mouse |
Alternative names: | EC 3.1.2.12 Es-10 Es10 ESD ESTD_HUMAN Esterase 10 Esterase D Esterase D formylglutathione hydrolase FGH FGH, included FLJ11763 Methylumbelliferyl acetate deacetylase MGC139609 OTTHUMP00000018374 OTTHUMP00000040929 S formylglutathione hydrolase S-formylglutathione hydrolase S-formylglutathione hydrolase, included Sid 478 sid478 |
Fig1: Western blot analysis of ESD on K562 cell (1) and mouse intestine tissue (2) lysate using anti-ESD antibody at 1/1,000 dilution. | |
Fig2: ICC staining ESD in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining ESD in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: ICC staining ESD in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-ESD antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ESD antibody. Counter stained with hematoxylin. | |
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-e=ESD antibody. Counter stained with hematoxylin. |