ESD Rabbit Polyclonal Antibody
cat.: R1511-9
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ESD aa 1-282 / 282. |
| Positive control: | K562 cell lysate, mouse intestine tissue lysate, Hela, LOVO, SW480, human prostate tissue, human pancreas tissue, mouse colon tissue. |
| Subcellular location: | Cytoplasm, Cytoplasmic vesicle. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:5000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P10768 Human | Q9R0P3 Mouse |
| Alternative names: | EC 3.1.2.12 Es-10 Es10 ESD ESTD_HUMAN Esterase 10 Esterase D Esterase D formylglutathione hydrolase FGH FGH, included FLJ11763 Methylumbelliferyl acetate deacetylase MGC139609 OTTHUMP00000018374 OTTHUMP00000040929 S formylglutathione hydrolase S-formylglutathione hydrolase S-formylglutathione hydrolase, included Sid 478 sid478 |
Images
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Fig1:
Western blot analysis of ESD on different lysates with Rabbit anti-ESD antibody (R1511-9) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ESD KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-9) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of ESD on K562 cell (1) and mouse intestine tissue (2) lysate using anti-ESD antibody at 1/1,000 dilution. |
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Fig3: ICC staining ESD in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining ESD in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining ESD in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-ESD antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ESD antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-e=ESD antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.