B7-H4(VTCN1) Rabbit Polyclonal Antibody
cat.: R1512-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human B7-H4 aa 65-226 / 282. |
| Positive control: | SK-Br-3 cell lysate, human liver tissue lysate, mouse testis tissue lysate, mouse spleen tissue lysate, Hela, MCF-7, SK-Br-3, human tonsil tissue, human lung cancer tissue, Jurkat. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q7Z7D3 Human | Q7TSP5 Mouse |
| Alternative names: | B7 family member, H4 B7 H4 B7 homolog 4 B7 superfamily member 1 B7 superfamily, member 1 B7-H4 B7h.5 B7h4 B7S1 B7x BC032925 Immune costimulatory protein B7-H4 Immune costimulatory protein B7H4 MGC41287 PRO1291 Protein B7S1 RP11 229A19.4 T cell costimulatory molecule B7x T-cell costimulatory molecule B7x V set domain-containing T cell activation inhibitor 1 V-set domain-containing T-cell activation inhibitor 1 VCTN1 Vtcn1 VTCN1_HUMAN |
Images
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Fig1:
Western blot analysis of B7-H4 on different lysate using anti-B7-H4 antibody at 1/1,000 dilution. Positive control: Lane 1: SK-Br-3 cell lysate Lane 2: Human liver tissue lysate Lane 3: Mouse testis tissue lysate Lane 4: Mouse spleen tissue lysate |
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Fig2: ICC staining B7-H4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining B7-H4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining B7-H4 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- B7-H4 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-B7-H4 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Jurkat cells with B7-H4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.