IMP3 Rabbit Polyclonal Antibody
cat.: R1512-10
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 64 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IMP-3 aa 352-579 / 579. |
| Positive control: | HeLa cell lysate, 293T cell lysate, MCF7 cell lysate, THP-1 cell lysate, rat brain tissue, human placental tissue, mouse testis tissue, mouse brain tissue, HepG2. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000-1:2,000 1:50-1:400 1:50-1:100 |
| Uniprot #: | SwissProt: O00425 Human | Q9CPN8 Mouse Entrez Gene: 312320 Rat |
| Alternative names: | Cancer/testis antigen 98 CT98 DKFZp686F1078 hKOC IF2B3_HUMAN IGF II mRNA binding protein 3 IGF-II mRNA-binding protein 3 IGF2 mRNA binding protein 3 IGF2 mRNA-binding protein 3 IGF2BP3 IMP 3 IMP-3 Insulin like growth factor 2 mRNA binding protein 3 Insulin-like growth factor 2 mRNA-binding protein 3 KH domain containing protein overexpressed in cancer KH domain-containing protein overexpressed in cancer KOC 1 KOC1 VICKZ 3 VICKZ family member 3 VICKZ3 |
Images
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Fig1:
Western blot analysis of IMP3 on different lysates with Rabbit anti-IMP3 antibody (R1512-10) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MCF7 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 64 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IMP-3 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human placental tissue using anti-IMP-3 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IMP-3 antibody. Counter stained with hematoxylin. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-IMP3 antibody (R1512-10) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1512-10) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of HepG2 cells with IPM-3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.