IMP3 Rabbit Polyclonal Antibody
cat.: R1512-10
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 64 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human IMP-3 aa 352-579 / 579.
Positive control: HeLa cell lysate, 293T cell lysate, MCF7 cell lysate, THP-1 cell lysate, rat brain tissue, human placental tissue, mouse testis tissue, mouse brain tissue, HepG2.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:1,000-1:2,000
1:50-1:400
1:50-1:100
Uniprot #: SwissProt: O00425 Human | Q9CPN8 Mouse
Entrez Gene: 312320 Rat
Alternative names: Cancer/testis antigen 98 CT98 DKFZp686F1078 hKOC IF2B3_HUMAN IGF II mRNA binding protein 3 IGF-II mRNA-binding protein 3 IGF2 mRNA binding protein 3 IGF2 mRNA-binding protein 3 IGF2BP3 IMP 3 IMP-3 Insulin like growth factor 2 mRNA binding protein 3 Insulin-like growth factor 2 mRNA-binding protein 3 KH domain containing protein overexpressed in cancer KH domain-containing protein overexpressed in cancer KOC 1 KOC1 VICKZ 3 VICKZ family member 3 VICKZ3
Images
R1512-10_1.jpg Fig1: Western blot analysis of IMP3 on different lysates with Rabbit anti-IMP3 antibody (R1512-10) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: MCF7 cell lysate
Lane 4: THP-1 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 64 kDa
Observed band size: 70 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
R1512-10_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IMP-3 antibody. Counter stained with hematoxylin.
R1512-10_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human placental tissue using anti-IMP-3 antibody. Counter stained with hematoxylin.
R1512-10_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IMP-3 antibody. Counter stained with hematoxylin.
R1512-10_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-IMP3 antibody (R1512-10) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1512-10) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1512-10_6.jpg Fig6: Flow cytometric analysis of HepG2 cells with IPM-3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.