| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human AKR1C1 aa 180-323 / 323. |
| Positive control: | Mouse testes tissue lysate, SW480 cell lysate, human liver cancer tissue, mouse kidney tissue, mouse stomach tissue, HepG2. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q04828 Human |
| Alternative names: | 2-dihydrobenzene-1 2-diol dehydrogenase 20 alpha (3 alpha) hydroxysteroid dehydrogenase 20 ALPHA HSD 20 alpha hydroxysteroid dehydrogenase 20-alpha-HSD 20-alpha-hydroxysteroid dehydrogenase 20ALPHAHSD 2ALPHAHSD AK1C1 AK1C1_HUMAN AKR1C1 Aldo keto reductase family 1 member C1 aldo-keto reductase C Aldo-keto reductase family 1 member C1 Aldo-keto reductase family, 1 member 1 C9 Chlordecone reductase homolog Chlordecone reductase homolog HAKRC DD1 DD1/DD2 DDH DDH1 Dihydrodiol dehydrogenase 1 Dihydrodiol dehydrogenase 1/2 dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase dihydrodiol dehydrogenase isoform DD1 Dihydrodiol dehydrogenase, type 1 H37 HAKRC HBAB Hepatic dihydrodiol dehydrogenase High affinity hepatic bile acid-binding protein High-affinity hepatic bile acid-binding protein Indanol dehydrogenase MBAB MGC8954 Trans-1 Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase Type II 3 alpha...... |
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Fig1:
Western blot analysis of AKR1C1 on different lysate using anti-AKR1C1 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse testis tissue lysate Lane 2: SW480 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin. |
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Fig5: Flow cytometric analysis of HepG2 cells with AKR1C1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). |