LDL Receptor Rabbit Polyclonal Antibody
cat.: R1512-12
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 95 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human LDL Receptor aa 377-441.
Positive control: Hela cell lysates, MCF-7 cell lysates, A549, PC-3M, human liver cancer tissue, mouse testis tissue, Hela.
Subcellular location: Golgi apparatus, Plasma membrane, Endosome, Lysosome, Cell membrane.
Recommended Dilutions:
  WB
  IF-cell
  FC
1:500-2000
1:100
1μg/Test
Uniprot #: SwissProt: P01130 Human | P35951 Mouse
Alternative names: FH FHC LDL R LDL receptor LDLCQ2 Ldlr LDLR_HUMAN Low Density Lipoprotein Receptor Low density lipoprotein receptor class A domain containing protein 3 Low density lipoprotein receptor familial hypercholesterolemia Low-density lipoprotein receptor
Images
R1512-12_1.jpg Fig1: Sample:Human colon Lysate at 45ug;
Primary: Anti-LDL receptor (bs-0705R) at 1:300 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 92kD
Observed band size : 92kD
R1512-12_2.jpg Fig2: Sample:
K562(Human) Cell Lysate at 30 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
SW480(Human) Cell Lysate at 30 ug
Primary: Anti-LDL receptor (bs-0705R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 92 kD
Observed band size: 120 kD
R1512-12_3.jpg Fig3: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (LDL receptor) polyclonal Antibody, Unconjugated (bs-0705R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
R1512-12_4.jpg Fig4: Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (bs-0705R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
R1512-12_5.jpg Fig5: Blank control (black line): HepG2 (black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature)
Primary Antibody (green line): Rabbit Anti-LDLreceptor antibody (bs-0705R) ;
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1μg /test.
R1512-12_6.jpg Fig6: Blank control：A431.
Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (bs-0705R),Dilution: 1μg /10^6 cells.
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody:Goat anti-rabbit IgG-AF647,Dilution: 1μg /test.
Protocol
A431 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.