LDL Receptor Rabbit Polyclonal Antibody
cat.: R1512-12
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 95 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human LDL Receptor aa 377-441. |
| Positive control: | Hela cell lysates, MCF-7 cell lysates, A549, PC-3M, human liver cancer tissue, mouse testis tissue, Hela. |
| Subcellular location: | Golgi apparatus, Plasma membrane, Endosome, Lysosome, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P01130 Human | P35951 Mouse |
| Alternative names: | FH FHC LDL R LDL receptor LDLCQ2 Ldlr LDLR_HUMAN Low Density Lipoprotein Receptor Low density lipoprotein receptor class A domain containing protein 3 Low density lipoprotein receptor familial hypercholesterolemia Low-density lipoprotein receptor |
Images
|
Fig1: Western blot analysis of LDL Receptor on Hela (1) and MCF-7 (2) cell lysates using anti-LDL Receptor antibody at 1/1,000 dilution. |
|
Fig2: ICC staining LDL Receptor in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining LDL Receptor in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin. |
|
Fig6: Flow cytometric analysis of Hela cells with LDL Receptor antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.