VCAM1 Rabbit Polyclonal Antibody
cat.: R1512-13
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 81 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human VCAM1aa 1-50 / 739. |
| Positive control: | Human spleen tissue lysate, Hela, JAR, SHG-44, human spleen tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:2,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P19320 Human |
| Alternative names: | CD106 CD106 Antigen INCAM 100 INCAM-100 L1CAM MGC99561 V-CAM 1 Vascular Cell Adhesion Molecule 1 Vascular cell adhesion protein 1 VCAM 1 VCAM-1 VCAM1 VCAM1_HUMAN |
Images
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Fig1: Western blot analysis of VCAM1 on human spleen tissue lysate using anti-VCAM1 antibody at 1/5,000 dilution. |
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Fig2: ICC staining VCAM1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining VCAM1 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining VCAM1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-VCAM1 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of Hela cells with VCAM1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.