ACO1 Rabbit Polyclonal Antibody
cat.: R1512-16
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 98 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ACO1 aa 1-273 / 889. |
| Positive control: | Mouse liver tissue lysate, rat liver tissue lysate, Hela, HepG2, human thyroid tissue, human kidney tissue, mouse thyroid tissue, mouse ovary tissue, mouse kidney tissue. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P21399 Human | P28271 Mouse | Q63270 Rat |
| Alternative names: | ACO 1 ACO1 ACOC_HUMAN Aconitase 1 soluble Aconitase Aconitase1 Aconitate hydratase ACONS Citrate hydro lyase Citrate hydro-lyase Cytoplasmic aconitate hydratase Ferritin repressor protein IRE BP 1 IRE-BP 1 IREB 1 IREB1 IREBP IREBP1 Iron regulatory protein 1 Iron responsive element binding protein 1 Iron-responsive element-binding protein 1 IRP 1 IRP1 OTTHUMP00000045233 |
Images
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Fig1:
Western blot analysis of ACO1 on different lysates with Rabbit anti-ACO1 antibody (R1512-16) at 1/2,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 98 kDa Observed band size: 98 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-16) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining ACO1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining ACO1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ACO1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACO1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-ACO1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-ACO1 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ACO1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.