WDR5 Rabbit Polyclonal Antibody
cat.: R1512-17
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within N-terminal human WDR5. |
| Positive control: | MCF-7 cell lysate, Hela, MCF-7. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P61964 Human |
| Alternative names: | 2410008O07Rik AA408785 AA960360 BIG 3 Big BIG3 BMP2 induced 3 kb gene protein BMP2 induced gene, 3-KB BMP2-induced 3-kb gene protein MGC114572 OTTHUMP00000162494 SWD 3 SWD3 SWD3 Set1c WD40 repeat protein homolog WD repeat containing protein 5 WD repeat domain 5 WD repeat protein 5 WD repeat-containing protein 5 WD repeat-containing protein BIG-3 WDR 5 Wdr5 WDR5_HUMAN |
Images
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Fig1: ICC staining WDR5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining WDR5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3:
Western blot analysis of WDR5 on different lysates with Rabbit anti-WDR5 antibody (R1512-17) at 1/1,000 dilution. Lane 1: HeLa cell lysate Exposure time: 119 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1512-17, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 37 kDa Observed band size: 37 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.