WDR5 Rabbit Polyclonal Antibody
cat.: R1512-17
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 37 kDa
Isotype: IgG
Immunogen: Recombinant protein within N-terminal human WDR5.
Positive control: MCF-7 cell lysate, Hela, MCF-7.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  FC
1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P61964 Human
Alternative names: 2410008O07Rik AA408785 AA960360 BIG 3 Big BIG3 BMP2 induced 3 kb gene protein BMP2 induced gene, 3-KB BMP2-induced 3-kb gene protein MGC114572 OTTHUMP00000162494 SWD 3 SWD3 SWD3 Set1c WD40 repeat protein homolog WD repeat containing protein 5 WD repeat domain 5 WD repeat protein 5 WD repeat-containing protein 5 WD repeat-containing protein BIG-3 WDR 5 Wdr5 WDR5_HUMAN
Images
R1512-17_1.jpg Fig1: Western blot analysis of WDR5 on MCF-7 cell lysate using anti-WDR5 antibody at 1/500 dilution.
R1512-17_2.jpg Fig2: ICC staining WDR5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1512-17_3.jpg Fig3: ICC staining WDR5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1512-17_4.jpg Fig4: Flow cytometric analysis of MCF-7 cells with WDR5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.