Myosin Light Chain 2 Rabbit Polyclonal Antibody
cat.: R1512-19
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 19 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Myosin Light Chain 2 aa 21-70 / 166.
Positive control: A431 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse heart tissue lysate, Mouse skeletal tissue lysate, Rat heart tissue lysate, A431, C2C12, C6, mouse heart tissue, rat heart tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:2,000-1:5,000
1:1,000
1:50-1:200
1:1,000
Uniprot #: SwissProt: P10916 Human | P51667 Mouse | P08733 Rat
Alternative names: Cardiac myosin light chain-2 Cardiac ventricular myosin light chain 2 CMH10 MLC 2v MLC-2 MLC-2v MLC2 MLRV_HUMAN MYL 2 MYL2 Myosin light chain 2 regulatory cardiac slow Myosin light polypeptide 2 regulatory cardiac slow Myosin regulatory light chain 2 Myosin regulatory light chain 2 ventricular/cardiac muscle isoform Regulatory light chain of myosin RLC of myosin Slow cardiac myosin regulatory light chain 2 ventricular/cardiac muscle isoform
Images
R1512-19_1.jpg Fig1: Western blot analysis of Myosin Light Chain 2 on different lysates with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/2,000 dilution.

Lane 1: A431 cell lysate (15 µg/Lane)
Lane 2: C2C12 cell lysate (15 µg/Lane)
Lane 3: C6 cell lysate (15 µg/Lane)
Lane 4: Mouse heart tissue lysate (20 µg/Lane)
Lane 5: Mouse skeletal tissue lysate (20 µg/Lane)
Lane 6: Rat heart tissue lysate (20 µg/Lane)

Predicted band size: 19 kDa
Observed band size: 19 kDa

Exposure time: 2 minutes 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-19) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
R1512-19_2.jpg Fig2: Immunocytochemistry analysis of A431 cells labeling Myosin Light Chain 2 with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
R1512-19_3.jpg Fig3: Immunocytochemistry analysis of C2C12 cells labeling Myosin Light Chain 2 with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1.000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
R1512-19_4.jpg Fig4: Immunocytochemistry analysis of C6 cells labeling Myosin Light Chain 2 with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
R1512-19_5.jpg Fig5: Flow cytometric analysis of A431 cells labeling Myosin Light Chain 2.

Cells were fixed and permeabilized. Then stained with the primary antibody (R1512-19, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
R1512-19_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
R1512-19_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.