Myosin Light Chain 2 Rabbit Polyclonal Antibody
cat.: R1512-19
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 19 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Myosin Light Chain 2 aa 21-70 / 166.
Positive control: A431 cell lysate, C2C12 cell lysate, C6 cell lysate, mouse skeletal tissue lysate, rat heart tissue lysate, A549, C2C12, mouse heart tissue, rat heart tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:5,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P10916 Human | P51667 Mouse | P08733 Rat
Alternative names: Cardiac myosin light chain-2 Cardiac ventricular myosin light chain 2 CMH10 MLC 2v MLC-2 MLC-2v MLC2 MLRV_HUMAN MYL 2 MYL2 Myosin light chain 2 regulatory cardiac slow Myosin light polypeptide 2 regulatory cardiac slow Myosin regulatory light chain 2 Myosin regulatory light chain 2 ventricular/cardiac muscle isoform Regulatory light chain of myosin RLC of myosin Slow cardiac myosin regulatory light chain 2 ventricular/cardiac muscle isoform
Images
R1512-19_1.jpg Fig1: Western blot analysis of Myosin Light Chain 2 on different lysates with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/5,000 dilution.

Lane 1: A431 cell lysate (15 µg/Lane)
Lane 2: C2C12 cell lysate (15 µg/Lane)
Lane 3: C6 cell lysate (15 µg/Lane)
Lane 4: Mouse skeletal tissue lysate (20 µg/Lane)
Lane 5: Rat heart tissue lysate (20 µg/Lane)

Predicted band size: 19 kDa
Observed band size: 19 kDa

Exposure time: 2 minutes 37 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-19) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
R1512-19_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
R1512-19_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
R1512-19_4.jpg Fig4: Flow cytometric analysis of Hela cells with Myosin Light Chain 2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
R1512-19_5.jpg Fig5: Immunocytochemistry analysis of A431 cells labeling Myosin Light Chain 2 with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
R1512-19_6.jpg Fig6: Immunocytochemistry analysis of C2C12 cells labeling Myosin Light Chain 2 with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1.000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
R1512-19_7.jpg Fig7: Immunocytochemistry analysis of C6 cells labeling Myosin Light Chain 2 with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
R1512-19_8.jpg Fig8: Flow cytometric analysis of A431 cells labeling Myosin Light Chain 2.

Cells were fixed and permeabilized. Then stained with the primary antibody (R1512-19, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.