Myosin Light Chain 2 Rabbit Polyclonal Antibody
cat.: R1512-19
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Myosin Light Chain 2 aa 21-70 / 166. |
| Positive control: | A431 cell lysate, C2C12 cell lysate, C6 cell lysate, mouse skeletal tissue lysate, rat heart tissue lysate, A549, C2C12, mouse heart tissue, rat heart tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P10916 Human | P51667 Mouse | P08733 Rat |
| Alternative names: | Cardiac myosin light chain-2 Cardiac ventricular myosin light chain 2 CMH10 MLC 2v MLC-2 MLC-2v MLC2 MLRV_HUMAN MYL 2 MYL2 Myosin light chain 2 regulatory cardiac slow Myosin light polypeptide 2 regulatory cardiac slow Myosin regulatory light chain 2 Myosin regulatory light chain 2 ventricular/cardiac muscle isoform Regulatory light chain of myosin RLC of myosin Slow cardiac myosin regulatory light chain 2 ventricular/cardiac muscle isoform |
Images
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Fig1:
Western blot analysis of Myosin Light Chain 2 on different lysates with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/5,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: C2C12 cell lysate (15 µg/Lane) Lane 3: C6 cell lysate (15 µg/Lane) Lane 4: Mouse skeletal tissue lysate (20 µg/Lane) Lane 5: Rat heart tissue lysate (20 µg/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-19) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining Myosin Light Chain 2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Myosin Light Chain 2 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of Hela cells with Myosin Light Chain 2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.