GPCR RDC1/CXCR-7 Rabbit Polyclonal Antibody
cat.: R1512-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C terminal human CXCR-7. |
| Positive control: | Hela cell lysate, MCF-7 cell lysate, Jurkat cell lysate, Hela, HUVEC, MCF-7. |
| Subcellular location: | Cell membrane, Cytoplasm, Endosome, Membrane |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P25106 Human |
| Alternative names: | ACKR3 atypical chemokine receptor 3 C X C chemokine receptor type 7 C-X-C chemokine receptor type 7 Chemokine (C-X-C motif) receptor 7 Chemokine C X C motif receptor 7 Chemokine orphan receptor 1 CMKOR1 CXC R7 CXC-R7 CXCR 7 CXCR-7 CXCR7 CXCR7_HUMAN G protein coupled receptor 159 G protein coupled receptor G protein coupled receptor RDC1 homolog G-protein coupled receptor 159 G-protein coupled receptor RDC1 homolog GPCR RDC1 GPR159 GPRN1 RDC 1 RDC-1 RDC1 |
Images
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Fig1:
Western blot analysis of GPCR RDC1/CXCR-7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1512-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: MCF-7 cell lysate Lane 3: Jurkat cell lysate |
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Fig2: ICC staining of GPCR RDC1/CXCR-7 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1512-3, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of GPCR RDC1/CXCR-7 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1512-3, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Flow cytometric analysis of GPCR RDC1/CXCR-7 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (R1512-3, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.