PLCG2 Rabbit Polyclonal Antibody
cat.: R1512-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 148 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PLCG2 aa 1200-1265. |
| Positive control: | Raji cell lysate, Daudi cell lysate, AGS, human tonsil tissue, Daudi. |
| Subcellular location: | Cytosol, Autolysosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:100 1:100-1:400 1:50-1:100 |
| Uniprot #: | SwissProt: P16885 Human |
| Alternative names: | 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase gamma-2 EC 3.1.4.11 Phosphoinositide phospholipase C Phosphoinositide phospholipase C-gamma-2 Phospholipase C gamma 2 Phospholipase C, gamma 2 (phosphatidylinositol specific) Phospholipase C-gamma-2 Phospholipase C-IV PLC 2 PLC gamma 2 PLC IV PLC-gamma-2 PLC-IV Plcg2 PLCG2_HUMAN |
Images
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Fig1:
Western blot analysis of PLCG2 on different lysates with Rabbit anti-PLCG2 antibody (R1512-4) at 1/1,000 dilution. Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 2: Daudi (Human Burkitt's lymphoma cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1512-4, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 148 kDa Observed band size: 148 kDa |
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Fig2: ICC staining of PLCG2 in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1512-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PLCG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1512-4, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of PLCG2 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (R1512-4, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.