Anti-PLCG2 antibody
cat.: R1512-4
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, IHC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 148 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human PLCG2 aa 1200-1265.
Positive control: Raji cell lysates, AGS, human tonsil tissue, Daudi.
Subcellular location: Cytosol, Autolysosome.
Recommended Dilutions:

Uniprot #: SwissProt: P16885 Human
Alternative names: 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase gamma-2 EC Phosphoinositide phospholipase C Phosphoinositide phospholipase C-gamma-2 Phospholipase C gamma 2 Phospholipase C, gamma 2 (phosphatidylinositol specific) Phospholipase C-gamma-2 Phospholipase C-IV PLC 2 PLC gamma 2 PLC IV PLC-gamma-2 PLC-IV Plcg2 PLCG2_HUMAN
R1512-4_1.jpg Fig1: Western blot analysis of PLCG2 on Raji cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1512-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
R1512-4_2.jpg Fig2: ICC staining of PLCG2 in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1512-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
R1512-4_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PLCG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1512-4, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
R1512-4_4.jpg Fig4: Flow cytometric analysis of PLCG2 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (R1512-4, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).