eIF-4E Rabbit Polyclonal Antibody
cat.: R1512-8
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human eIF-4E aa 1168-217 / 217. |
| Positive control: | PC-12, NIH-3T3, A431, Hela, SK-Br-3, 293T, human colon cancer tissue, mouse colon tissue, mouse brain tissue, mouse hippocampus tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P06730 Human | P63073 Mouse | P63074 Rat |
| Alternative names: | AUTS19 CBP eIF 4E eIF 4F 25 kDa subunit EIF 4F eIF-4E eIF-4F 25 kDa subunit eIF4E EIF4E1 EIF4EL1 EIF4F Eukaryotic translation initiation factor 4 E Eukaryotic translation initiation factor 4E Eukaryotic translation initiation factor 4E like 1 IF4E_HUMAN Messanger RNA Cap Binding Protein eIF 4E MGC111573 mRNA cap binding protein mRNA cap-binding protein |
Images
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Fig1:
Western blot analysis of eIF-4E on different cell lysates using anti-eIF-4E antibody at 1/500 dilution. Positive control: Lane 1: PC-12 Lane 2: NIH-3T3 |
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Fig2: ICC staining eIF-4E in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining eIF-4E in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining eIF-4E in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-eIF-4E antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-eIF-4E antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-eIF-4E antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using anti-eIF-4E antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of 293T cells with eIF-4E antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.