Cathepsin D Rabbit Polyclonal Antibody
cat.: R1512-9
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cathepsin D aa 373-412 / 412. |
| Positive control: | MCF-7 cell lysate, Hela, HepG2, MCF-7, human liver tissue, human lung tissue. |
| Subcellular location: | Lysosome, Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P07339 Human | P18242 Mouse |
| Alternative names: | CatD CATD_HUMAN Cathepsin D Cathepsin D heavy chain CD Ceroid lipofuscinosis neuronal 10 CLN10 CPSD ctsd Epididymis secretory sperm binding protein Li 130P HEL S 130P Lysosomal aspartyl peptidase Lysosomal aspartyl protease MGC2311 |
Images
|
Fig1: Western blot analysis of Cathepsin D on MCF-7 cell lysate using anti-Cathepsin D antibody at 1/1,000 dilution. |
|
Fig2: ICC staining Cathepsin D in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining Cathepsin D in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: ICC staining Cathepsin D in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin. |
|
Fig7: Flow cytometric analysis of HepG2 cells with Cathepsin D antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
|
Fig8: Flow cytometric analysis of MCF-7 cells with Cathepsin D antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.