AFP Rabbit Polyclonal Antibody
cat.: R1601-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within N-terminal human AFP. |
| Positive control: | MCF-7 cell lysate, PC-12 cell lysate, human liver tissue lysate, HepG2, MCF-7, Hela, human liver cancer tissue, human breast cancer tissue, 293T. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P02771 Human | P02772 Mouse | P02773 Rat |
| Alternative names: | Afp AFPD Alpha fetoglobulin Alpha fetoprotein Alpha fetoprotein precursor Alpha-1-fetoprotein Alpha-fetoglobulin Alpha-fetoprotein alpha-fetoprotein, Hereditary persistence of, included FETA FETA_HUMAN Hereditary persistence of alpha fetoprotein HPAFP |
Images
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Fig1:
Western blot analysis of AFP on different lysates using anti-AFP antibody at 1/1,000 dilution. Postive control: Lane 1: MCF-7 cell lysate Lane 2: PC-12 cell lysate Lane 3: Human liver tissue lysate |
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Fig2: ICC staining AFP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining AFP in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining AFP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AFP antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-AFP antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of 239T cells with AFP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.