Hsc70 Rabbit Polyclonal Antibody
cat.: R1601-10
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Hsc70 aa 446-629. |
| Positive control: | Hela cell lysate, A431 cell lysate, NIH-3T3 cell lysate, PC-12 cell lysate, Hela, A549, SK-Br-3, human tonsil tissue, human colon cancer tissue, human breast tissue, human kidney tissue, mouse testis tissue, mouse prostate tissue, Jurkat. |
| Subcellular location: | Cell membrane, Cytoplasm, Membrane, Nucleus, Spliceosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P11142 Human | P63017 Mouse | P63018 Rat |
| Alternative names: | 2410008N15Rik Constitutive heat shock protein 70 Epididymis luminal protein 33 Epididymis secretory sperm binding protein Li 72p Heat shock 70 kDa protein 8 Heat shock 70kD protein 10 Heat shock 70kD protein 8 Heat shock 70kDa protein 8 Heat shock cognate 71 kDa protein Heat shock cognate protein 54 Heat shock cognate protein 71 kDa Heat shock protein 8 Heat shock protein A8 Heat shock protein family A (Hsp70) member 8 Heat-shock70-KD protein 10, formerly HEL 33 HEL S 72p HSC54 HSC71 Hsc73 HSP71 HSP73 HSP7C_HUMAN HSPA10 HSPA8 LAP1 Lipopolysaccharide associated protein 1 LPS associated protein 1 LPS associated protein MGC102007 MGC106514 MGC114311 MGC118485 MGC131511 MGC29929 N-myristoyltransferase inhibitor protein 71 NIP71 |
Images
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Fig1:
Western blot analysis of Hsc70 on different cell lysate using anti-Hsc70 antibody at 1/2,000 dilution. Positive control: Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lane 3: NIH-3T3 cell lysate Lane 4: PC-12 cell lysate |
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Fig2: ICC staining Hsc70 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Hsc70 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Hsc70 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Hsc70 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Hsc70 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Hsc70 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Hsc70 antibody. Counter stained with hematoxylin. |
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Fig9: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Hsc70 antibody. Counter stained with hematoxylin. |
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Fig10: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Hsc70 antibody. Counter stained with hematoxylin. |
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Fig11: Flow cytometric analysis of Jurkat cells with Hsc70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.