| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HLA Class 1 ABC aa 125-325 / 362. |
| Positive control: | Hela cell lysate, human colon cancer tissue, human prostate tissue, Jurkat. |
| Subcellular location: | Cell membrane, Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:200-1:500 1:50-1:200 |
| Uniprot #: | SwissProt: P30443 Human | P01889 Human | P30499 Human |
| Alternative names: | HLA A HLA B HLA C HLA class 1 A HLA class 1 B HLA class 1 C Major histocompatibility complex, class I, A + B + C MHC class I HLA A MHC class I HLA B MHC class I HLA C MHC HLA ABC |
|
Fig1: Western blot analysis of HLA Class 1 ABC on Hela cell lysate using anti-HLA Class 1 ABC antibody at 1/1,000 dilution. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HLA Class 1 ABC antibody. Counter stained with hematoxylin. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-HLA Class 1 ABC antibody. Counter stained with hematoxylin. |
|
Fig4: Flow cytometric analysis of Jurkat cells with HLA Class 1 ABC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |