Cathepsin D Rabbit Polyclonal Antibody
cat.: R1601-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal human Cathepsin D. |
| Positive control: | PC-12 cell lysate, MCF-7 cell lysate, NIH-3T3 cell lysate, Jurkat cell lysate, rat liver tissue, human liver tissue, mouse brain tissue, A549. |
| Subcellular location: | Lysosome, Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P07339 Human | P18242 Mouse | P24268 Rat |
| Alternative names: | CatD CATD_HUMAN Cathepsin D Cathepsin D heavy chain CD Ceroid lipofuscinosis neuronal 10 CLN10 CPSD ctsd Epididymis secretory sperm binding protein Li 130P HEL S 130P Lysosomal aspartyl peptidase Lysosomal aspartyl protease MGC2311 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin. |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin. |
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Fig4:
Western blot analysis of Cathepsin D on different lysates with Rabbit anti-Cathepsin D antibody (R1601-2) at 1/1,000 dilution. Lane 1: C6 (Rat glioma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 7 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1601-2, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45 kDa Observed band size: 45 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.