Filamin-A Rabbit Polyclonal Antibody
cat.: R1601-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 281 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Filamin-A aa 2,373-2,619 / 2,647. |
| Positive control: | Hela cell lysate, NIH-3T3 cell lysate, Hela, HUVEC, MCF-7, human uterus tissue, human stomach cancer tissue. |
| Subcellular location: | Cytoplasm, cytoskeleton, Cell projection. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P21333 Human | Q8BTM8 Mouse |
| Alternative names: | ABP 280 ABP-280 Actin-binding protein 280 Alpha filamin Alpha-filamin APBX CSBS CVD1 Endothelial actin binding protein Endothelial actin-binding protein Filamin 1 Filamin A alpha Filamin A Filamin-1 Filamin-A FLN FLN-A FLN1 FLNA FLNA_HUMAN FMD MNS NHBP Non muscle filamin Non-muscle filamin OPD OPD1 OPD2 XLVD XMVD |
Images
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Fig1: Western blot analysis of Filamin-A on Hela (1) and NIH-3T3 (2) cell lysates using anti-Filamin-A antibody at 1/5,000 dilution. |
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Fig2: ICC staining Filamin-A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Filamin-A in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Filamin-A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Filamin-A antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Filamin-A antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Hela cells with Filamin-A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.