HDAC2 Rabbit Polyclonal Antibody
cat.: R1601-7
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal human Histone Deacetylase 2. |
| Positive control: | SH-SY5Y cell lysate, 293T cell lysate, Hela cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, COS-7 cell lysate, Wild-type Hela whole cell lysate, LOVO, NIH/3T3, SH-SY5Y, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse brain tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:10,000 1:500-1:2,000 1:100-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q92769 Human | P70288 Mouse | B1WBY8 Rat |
| Alternative names: | D10Wsu179e HD 2 HD2 HDAC 2 Hdac2 HDAC2_HUMAN Histone deacetylase 2 (HD2) Histone deacetylase 2 OTTHUMP00000017046 OTTHUMP00000227077 OTTHUMP00000227078 RPD3 transcriptional regulator homolog RPD3 YAF1 YY1 associated factor 1 YY1 transcription factor binding protein Yy1bp |
Images
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Fig1:
Western blot analysis of HDAC2 on different lysates with Rabbit anti-HDAC2 antibody (R1601-7) at 1/10,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 2: 293T (Human embryonic kidney cell) cell lysate Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 5: NIH/3T3 (Mouse fibroblast) cell lysate Lane 6: COS-7 (African green monkey kidney fibroblast) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1601-7, 1/10,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 55 kDa |
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Fig2:
All lanes: Western blot analysis of HDAC2 with anti-HDAC2 antibody (R1601-7) at 1/500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2/3: HDAC2 knockout Hela whole cell lysate (10 µg). R1601-7 was shown to specifically react with HDAC2 in wild-type Hela cells. No bands were observed when HDAC2 knockout sample were tested. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1601-7, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining HDAC2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining HDAC2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining HDAC2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HDAC2 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HDAC2 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC2 antibody. Counter stained with hematoxylin. |
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Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HDAC2 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.