ASS1 Rabbit Polyclonal Antibody
cat.: R1608-5
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ASS1 aa 1-208 / 412. |
| Positive control: | Hela, MCF-7, A549, NIH-3T3, Jurkat, human liver tissue, human kidney tissue, 2 liver tissue, 2 kidney tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:100-1:500 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P00966 Human | P16460 Mouse | P09034 Rat |
| Alternative names: | Argininosuccinate synthase 1 Argininosuccinate synthase Argininosuccinate synthetase 1 ASS Ass-1 ass1 ASSA ASSY_HUMAN Citrulline aspartate ligase Citrulline--aspartate ligase CTLN1 |
Images
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Fig1: Western blot analysis of ASS1 on Hela (1) and MCF-7 (2) cell lysate using anti-ASS1 antibody at 1/1,000 dilution. |
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Fig2: ICC staining ASS1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining ASS1 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ASS1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ASS1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded 2 liver tissue using anti-ASS1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded 2 kidney tissue using anti-ASS1 antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of Jurkat cells with ASS1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.