ASS1 Rabbit Polyclonal Antibody
cat.: R1608-5
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ASS1 aa 1-208 / 412. |
| Positive control: | Hela, MCF-7, A549, NIH-3T3, Jurkat, human liver tissue, human kidney tissue, mouse liver tissue, mouse kidney tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:100-1:500 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P00966 Human | P16460 Mouse | P09034 Rat |
| Alternative names: | Argininosuccinate synthase 1 Argininosuccinate synthase Argininosuccinate synthetase 1 ASS Ass-1 ass1 ASSA ASSY_HUMAN Citrulline aspartate ligase Citrulline--aspartate ligase CTLN1 |
Images
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Fig1: ICC staining ASS1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining ASS1 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ASS1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ASS1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ASS1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ASS1 antibody. Counter stained with hematoxylin. |
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Fig7:
Western blot analysis of ASS1 on different lysates with Rabbit anti-ASS1 antibody (R1608-5) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1608-5, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 47 kDa Observed band size: 47 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.