PCBP1 Rabbit Polyclonal Antibody
cat.: R1608-6
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PCBP1 aa aa 1-162 / 356. |
| Positive control: | F9, NIH/3T3, K562, A549, HepG2, MCF-7, human spleen tissue, mouse testis tissue, rat brain tissue. |
| Subcellular location: | Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:200-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q15365 Human | P60335 Mouse | A0A8I6AH91 Rat |
| Alternative names: | Alpha-CP1 Heterogeneous nuclear ribonucleoprotein E1 heterogenous nuclear ribonucleoprotein E1 heterogenous nuclear ribonucleoprotein X hnRNP E1 hnRNP-E1 hnRNP-X HNRPE1 HNRPX nucleic acid binding protein sub 2.3 Nucleic acid- binding protein SUB2.3 Nucleic acid-binding protein SUB2.3 PCBP1 PCBP1_HUMAN poly(rC) binding protein 1 Poly(rC)-binding protein 1 rCbinding protein 1 |
Images
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Fig1:
Western blot analysis of PCBP1 on different cell lysate using anti-PCBP1 antibody at 1/1,000 dilution. Positive control: Lane 1: F9 Lane 2: NIH/3T3 Lane 3: K562 |
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Fig2: ICC staining PCBP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining PCBP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining PCBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PCBP1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PCBP1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PCBP1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.