| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 86 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Glucocorticoid Receptor alpha aa 31-390. |
| Positive control: | Hela, 293, A431. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:200-1:500 1:100-1:500 |
| Uniprot #: | SwissProt: P04150 Human | P06537 Mouse | P06536 Rat |
| Alternative names: | GCCR GCR Glucocorticoid receptor alpha isoform Glucocorticoid receptor GR GRL NR3C1 Nuclear receptor subfamily 3 group C member 1 |
|
Fig1: Western blot analysis of Glucocorticoid Receptor alpha on Hela cell lysate using anti-Glucocorticoid Receptor alpha antibody at 1/1,000 dilution. |
|
Fig2: ICC staining Glucocorticoid Receptor alpha in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining Glucocorticoid Receptor alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |